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Journal: Cancer Reports
Article Title: Integrated Transcriptomic Analysis Identifies ENO3 as a Candidate Lactate Metabolism‐Associated Biomarker in Colorectal Cancer Metastasis
doi: 10.1002/cnr2.70723
Figure Lengend Snippet: ENO3 is associated with CRC prognosis and contributes to tumor‐cell phenotypes. (A) Violin plot showing the differential expression of ENO3 in CRC tumor tissues compared to adjacent normal tissues, utilizing data from the TCGA COADREAD cohort. Statistical significance was determined by a two‐tailed Wilcoxon rank‐sum test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Kaplan–Meier survival curve illustrating the OS of CRC patients stratified by high and low ENO3 expression levels (grouped by the median expression value). The p ‐value was calculated using the log‐rank test. (C) CCK‐8 cell proliferation curve comparing the viability of MC38 wild‐type (WT) and ENO3 knockout (KO) cells over a period of 96 h. Data are presented as mean ± SD from n = 3 biological replicates. Statistical analysis was performed using two‐way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) Hematoxylin and eosin (H&E) staining of lung tissues from NSG mice inoculated by tail‐vein injection with MC38 WT or ENO3‐KO cells, showing pulmonary lesions in the experimental lung colonization model. Representative images are shown at ×100 magnification. Scale bar, 100 μm. (E) Quantification of pulmonary tumor nodules in NSG mice inoculated with MC38 WT or ENO3‐KO cells. Data are presented as mean ± SD. Each dot represents an individual mouse ( n = 5 mice per group). Statistical significance was determined using a two‐tailed unpaired t ‐test.
Article Snippet: Proliferation rates were quantified using
Techniques: Quantitative Proteomics, Two Tailed Test, Expressing, CCK-8 Assay, Knock-Out, Analysis, Staining, Injection